Johns Hopkins School of Medicine

To whom correspondence should be addressed. E-mail : clee75(at).jhmi.edu

In biochemistry and molecular biology, fusion protein technique allows us to isolate a protein of interest with easier purification methods or to identify the tagged protein even when any antibody of the target protein is yet developed. Green fluorescent protein (GFP) and its derivatives have been used as a fusion tag for many proteins in living cells and many in vivo experiments due to its convenience of non-invasive monitoring. Likewise, fluorescent proteins can be advantageous when they are being used as fusion tag for recombinant proteins in vitro. This paper introduces the approaches to take advantage of GFP variants as a fusion tag for in vitro experiments.

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