ABM (Applied Biological Materials,Inc.)    Origin : Canada

SafeView™ Nucleic Acid Stain

Description  SafeViewTM products represent a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic Ethidium Bromide (EB, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. SafeViewTM products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.   SafeViewTM Classic - Substitute for Ethidium Bromide in agarose gel SafeViewTM Classic is used the same way as Ethidium Bromide in agarose gel electrophoresis. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This stain has two fluorescence excitation maxima when bound to nucleic acid, at approximately 290nm and 490nm. Protocol 1. Prepare a 100ml agarose solution. 2. Add 5 μl SafeViewTM Classic to the agarose solution. 3. Mix gently; solution should have no air bubbles. 4. Let the solution cool down to 60-70oC and cast the gel. 5. Run gel electrophoresis with 5μl SafeViewTM classic per 100ml buffer. 6. View the results under UV light.   Safe-Green,Red,White,PackTM - Substitute for loading dye With SafeViewTM dyes (Safe-Green,Red,White,PackTM), you do not need to add any dyes to both gel matrix and running buffers. SafeViewTM dyes are provided in a form of 6x sample loading dyes and they are to be added to your samples only. The Safeview dyes completely eliminate any possible contamination of glassware or gel running tank as associated with EB. After the electrophoresis, view and document your results as you would do with EB staining protocols. Protocol 1. Prepare a 100ml agarose solution. 2. Mix gently without having any air bubbles. 3. Let the solution cool down to 60-70oC and cast the gel. 4. Mix samples and DNA markers with a SafeViewTM dye at 1:5 dilution rate. 5. View the results under UV light after electrophoresis.     Application  Safe Detection of dsDNA, ssDNA and RNA in agarose gels.

Data     MSDS:   (SafeView Classic) (SafeWhite) (SafeRed) (SafeGreen)

Notes  SafeViewTM Nucleic Acid Stains are non-carcinogenic, but may cause skin and eye irritaions. Always wear gloves when working with the product.

Cat. No. Product Name Size/Unit G108 SafeView™ Classic 1.0ml G108-G Safe-Green™ 1.0ml G108-R Safe-Red™ 1.0ml G108-W Safe-White™ 1.0ml G108-P Safe-Pack™ G108-W,G,R